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CIVMs derived from neonatal testis from PND 5 and PND 10 testis were exposure to isotretinoin for 24 hours. (A) Expression of Stra8 in PND 5 derived CIVMs determined by RT-qPCR. In the control, 0.3, 3, and 30 nM isotretinoin dose groups the expression of Stra8 contained samples below the threshold of quantification. The expression of Stra8 was significantly increased in the 300 nM isotretinoin dose group (p-value <0.0001). (B) Expression of <t>Plzf</t> in PND 5 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 300 nM isotretinoin dose group was significantly lower than control (p-value of 0.026). (C) Expression of Stra8 in PND 10 derived CIVMs determined by RT-qPCR. Aside from one control sample, all samples were above the threshold of quantification. The expression of Stra8 was significantly increased in the 3,30, and 300 nM isotretinoin dose groups (p-values all <0.0001). (D) Expression of Plzf in PND 10 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 30 and 300nM isotretinoin dose groups were significantly lower than control (p-values <0.0001).
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CIVMs derived from neonatal testis from PND 5 and PND 10 testis were exposure to isotretinoin for 24 hours. (A) Expression of Stra8 in PND 5 derived CIVMs determined by RT-qPCR. In the control, 0.3, 3, and 30 nM isotretinoin dose groups the expression of Stra8 contained samples below the threshold of quantification. The expression of Stra8 was significantly increased in the 300 nM isotretinoin dose group (p-value <0.0001). (B) Expression of <t>Plzf</t> in PND 5 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 300 nM isotretinoin dose group was significantly lower than control (p-value of 0.026). (C) Expression of Stra8 in PND 10 derived CIVMs determined by RT-qPCR. Aside from one control sample, all samples were above the threshold of quantification. The expression of Stra8 was significantly increased in the 3,30, and 300 nM isotretinoin dose groups (p-values all <0.0001). (D) Expression of Plzf in PND 10 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 30 and 300nM isotretinoin dose groups were significantly lower than control (p-values <0.0001).
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CIVMs derived from neonatal testis from PND 5 and PND 10 testis were exposure to isotretinoin for 24 hours. (A) Expression of Stra8 in PND 5 derived CIVMs determined by RT-qPCR. In the control, 0.3, 3, and 30 nM isotretinoin dose groups the expression of Stra8 contained samples below the threshold of quantification. The expression of Stra8 was significantly increased in the 300 nM isotretinoin dose group (p-value <0.0001). (B) Expression of <t>Plzf</t> in PND 5 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 300 nM isotretinoin dose group was significantly lower than control (p-value of 0.026). (C) Expression of Stra8 in PND 10 derived CIVMs determined by RT-qPCR. Aside from one control sample, all samples were above the threshold of quantification. The expression of Stra8 was significantly increased in the 3,30, and 300 nM isotretinoin dose groups (p-values all <0.0001). (D) Expression of Plzf in PND 10 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 30 and 300nM isotretinoin dose groups were significantly lower than control (p-values <0.0001).
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CIVMs derived from neonatal testis from PND 5 and PND 10 testis were exposure to isotretinoin for 24 hours. (A) Expression of Stra8 in PND 5 derived CIVMs determined by RT-qPCR. In the control, 0.3, 3, and 30 nM isotretinoin dose groups the expression of Stra8 contained samples below the threshold of quantification. The expression of Stra8 was significantly increased in the 300 nM isotretinoin dose group (p-value <0.0001). (B) Expression of <t>Plzf</t> in PND 5 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 300 nM isotretinoin dose group was significantly lower than control (p-value of 0.026). (C) Expression of Stra8 in PND 10 derived CIVMs determined by RT-qPCR. Aside from one control sample, all samples were above the threshold of quantification. The expression of Stra8 was significantly increased in the 3,30, and 300 nM isotretinoin dose groups (p-values all <0.0001). (D) Expression of Plzf in PND 10 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 30 and 300nM isotretinoin dose groups were significantly lower than control (p-values <0.0001).
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CIVMs derived from neonatal testis from PND 5 and PND 10 testis were exposure to isotretinoin for 24 hours. (A) Expression of Stra8 in PND 5 derived CIVMs determined by RT-qPCR. In the control, 0.3, 3, and 30 nM isotretinoin dose groups the expression of Stra8 contained samples below the threshold of quantification. The expression of Stra8 was significantly increased in the 300 nM isotretinoin dose group (p-value <0.0001). (B) Expression of <t>Plzf</t> in PND 5 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 300 nM isotretinoin dose group was significantly lower than control (p-value of 0.026). (C) Expression of Stra8 in PND 10 derived CIVMs determined by RT-qPCR. Aside from one control sample, all samples were above the threshold of quantification. The expression of Stra8 was significantly increased in the 3,30, and 300 nM isotretinoin dose groups (p-values all <0.0001). (D) Expression of Plzf in PND 10 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 30 and 300nM isotretinoin dose groups were significantly lower than control (p-values <0.0001).
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CIVMs derived from neonatal testis from PND 5 and PND 10 testis were exposure to isotretinoin for 24 hours. (A) Expression of Stra8 in PND 5 derived CIVMs determined by RT-qPCR. In the control, 0.3, 3, and 30 nM isotretinoin dose groups the expression of Stra8 contained samples below the threshold of quantification. The expression of Stra8 was significantly increased in the 300 nM isotretinoin dose group (p-value <0.0001). (B) Expression of Plzf in PND 5 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 300 nM isotretinoin dose group was significantly lower than control (p-value of 0.026). (C) Expression of Stra8 in PND 10 derived CIVMs determined by RT-qPCR. Aside from one control sample, all samples were above the threshold of quantification. The expression of Stra8 was significantly increased in the 3,30, and 300 nM isotretinoin dose groups (p-values all <0.0001). (D) Expression of Plzf in PND 10 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 30 and 300nM isotretinoin dose groups were significantly lower than control (p-values <0.0001).

Journal: bioRxiv

Article Title: Differential retinoic acid responses across testicular development in vitro

doi: 10.64898/2025.12.30.696417

Figure Lengend Snippet: CIVMs derived from neonatal testis from PND 5 and PND 10 testis were exposure to isotretinoin for 24 hours. (A) Expression of Stra8 in PND 5 derived CIVMs determined by RT-qPCR. In the control, 0.3, 3, and 30 nM isotretinoin dose groups the expression of Stra8 contained samples below the threshold of quantification. The expression of Stra8 was significantly increased in the 300 nM isotretinoin dose group (p-value <0.0001). (B) Expression of Plzf in PND 5 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 300 nM isotretinoin dose group was significantly lower than control (p-value of 0.026). (C) Expression of Stra8 in PND 10 derived CIVMs determined by RT-qPCR. Aside from one control sample, all samples were above the threshold of quantification. The expression of Stra8 was significantly increased in the 3,30, and 300 nM isotretinoin dose groups (p-values all <0.0001). (D) Expression of Plzf in PND 10 derived CIVMs determined by RT-qPCR. All samples were above the threshold of quantification. The expression of Plzf in the 30 and 300nM isotretinoin dose groups were significantly lower than control (p-values <0.0001).

Article Snippet: RT-qPCR was used to target the rat genes Stra8 (Rn01747849_m1) and Plzf (Rn01418644_m1).

Techniques: Derivative Assay, Expressing, Quantitative RT-PCR, Control

The expression of Plzf and Stra8 quantified by RT-qPCR at day in vitro 4 and day in vitro 15 in PND 5 tissue CIVMs and PND 10 tissue CIVMs in control and media supplementation conditions. Color indicates day in vitro , dashed line divides plot by PND 5 CIVMs and PND 10 CIVMs. Points that are “X” indicate samples that were below the limit of quantification (cycle threshold of 37). Data displayed as expression relative to beta-actin (housekeeping gene (HK), (2^ [gene – HK])).

Journal: bioRxiv

Article Title: Differential retinoic acid responses across testicular development in vitro

doi: 10.64898/2025.12.30.696417

Figure Lengend Snippet: The expression of Plzf and Stra8 quantified by RT-qPCR at day in vitro 4 and day in vitro 15 in PND 5 tissue CIVMs and PND 10 tissue CIVMs in control and media supplementation conditions. Color indicates day in vitro , dashed line divides plot by PND 5 CIVMs and PND 10 CIVMs. Points that are “X” indicate samples that were below the limit of quantification (cycle threshold of 37). Data displayed as expression relative to beta-actin (housekeeping gene (HK), (2^ [gene – HK])).

Article Snippet: RT-qPCR was used to target the rat genes Stra8 (Rn01747849_m1) and Plzf (Rn01418644_m1).

Techniques: Expressing, Quantitative RT-PCR, In Vitro, Control